A new study has created a highly selective inhibitor for Matrix Metallopeptidase 7 (MMP7), an enzyme important for cancer spread and progression. Using advanced peptide discovery technology, researchers identified a novel peptide, D'20, that specifically binds to and blocks MMP7 while leaving similar enzymes unaffected. This selectivity reduces potential side effects and enhances therapeutic effectiveness. D'20 also demonstrated remarkable stability in human blood and digestive environments, ensuring its activity in the body. By inhibiting MMP7, which plays a crucial role in cancer metastasis, D'20 positions itself as a promising candidate for future cancer treatments, paving the way for more targeted therapies for aggressive cancers, such as pancreatic cancer.
A team of researchers from the Hebrew University of Jerusalem, the Weizmann Institute of Science, and the University of Tokyo has made a significant breakthrough in the fight against cancer by developing a highly selective inhibitor for an enzyme called Matrix Metallopeptidase 7 (MMP7). This enzyme plays a crucial role in cancer progression, especially in helping tumors invade surrounding tissues and spread to other parts of the body (metastasis). MMP7 has long been recognized as an attractive target for cancer therapies, but creating drugs that specifically block it has proven challenging due to the structural similarities it shares with other related enzymes in the matrix metalloproteinase family. These enzymes have overlapping functions, which makes it difficult to design a drug that targets only MMP7 without affecting others.
Led by Professor Norman Metanis and PhD student Hiba Ghareeb from the Hebrew University, in collaboration with Professor Irit Sagi from the Weizmann Institute of Science and Professor Hiroaki Suga from the University of Tokyo, the study utilized a pioneering approach called Mirror-Image Random Nonstandard Peptide Integrated Discovery (MI-RaPID). This advanced technology enabled the research team to identify a new class of molecules—known as macrocyclic peptides—that bind specifically to MMP7, inhibiting its activity without interfering with similar enzymes.
One of the standout peptides discovered in this study, named D'20, was designed in a unique mirror-image form. This peptide is made up of twelve specially modified building blocks called D-amino acids, along with other structural elements, making it both stable and highly specific. In laboratory tests, D'20 demonstrated impressive ability to block MMP7 activity with a high degree of precision, without affecting other enzymes that have similar functions. Furthermore, D'20 was shown to stop the movement of pancreatic cancer cells, a critical step in halting metastasis, while leaving the normal growth of the cells unaffected.
The stability of D'20 was also remarkable—it retained its structure and function in human blood and in conditions that simulate the digestive system, suggesting it has great potential for further development as a drug. This level of stability is key in ensuring that the peptide can work effectively in the human body over time.
This research not only offers new hope for targeting MMP7 in cancer therapy but also showcases the power of mirror-image random peptide discovery technology in developing new treatments. By creating highly specific and stable peptides, the team has opened up exciting possibilities for treating aggressive cancers, like pancreatic cancer, and improving the outlook for patients facing these difficult diseases.
The research paper titled “Mirror-Image Random Nonstandard Peptide Integrated Discovery (MI-RaPID) Technology Yields Highly Stable and Selective Macrocyclic Peptide Inhibitors for Matrix Metallopeptidase 7” is now available at Angewandte Chemie and can be accessed at https://doi.org/10.1002/anie.202414256.
MI-RaPID Method
Description: MI-RaPID Method provides highly stabilized peptide inhibitors for inhibiting Cancer metastasis
Credit: Authors. Published by Angewandte Chemie
Researchers:
Hiba Ghareeb1, Choi Yi Li2, Anjana Shenoy3, Naama Rotenberg4, Julia M Shifman4, Takayuki Katoh2, Irit Sagi3, Hiroaki Suga2 and Norman Metanis1
Institution:
- The Hebrew University of Jerusalem, Institute of Chemistry
- The University of Tokyo, Department of Chemistry, Graduate School of Science
- Weizmann Institute of Science, Department of Immunology and Regenerative Biology
- The Hebrew University of Jerusalem, Department of Biological Chemistry, The Alexander Silverman Institute of Life Science
The Hebrew University of Jerusalem is Israel’s premier academic and research institution. With over 23,000 students from 90 countries, it is a hub for advancing scientific knowledge and holds a significant role in Israel’s civilian scientific research output, accounting for nearly 40% of it and has registered over 11,000 patents. The university’s faculty and alumni have earned eight Nobel Prizes, two Turing Awards a Fields Medal, underscoring their contributions to ground-breaking discoveries. In the global arena, the Hebrew University ranks 81st according to the Shanghai Ranking. To learn more about the university’s academic programs, research initiatives, and achievements, visit the official website at http://new.huji.ac.il/en
